Geneticists Perfect New Hybridization (Continued From Page 8) ir present concepts of world tod production to be changed larkedly. The biologists emphasize that se of parasexual hybridization i produce new crop varieties LANCASTER LABORATORIES, INC. ANALYTICAL SERVICES DIVISION Feeds, Flour, Forages, Foods Dairy Products, Water, Waste Water Bacteriological, Physical, Chemcial 2425 New Holland Pike Lancaster, Pa. 17601 Telephone (717)656-9043 or (717)656-9868 Red Fever Demo Day: Come and catch it! A new Kind of fever has hit the country. It began last summer with the introduction of IH’s new line of farm tractors. An interest and enthusiasm began to build that is un paralleled in our 50 years of producing FARMALL® tractors. And we’d like to show you why. By letting you test drive some of our new tractors. Under field conditions. Discover the superior workpower our engines provide. How the proven Hydrostatic transmission can improve your productivity. And how our quieter cabs can add to your comfort and reduce fatigue. Bring along-the cap you’ve been wearing in the field. (No matter whose insignia is on it.) We’ll trade you our new one for it free. Even if you’re not ready to pick up a new tractor. Pick up a new cap. ■ m rorS fouEr red fever reu it;vci It’s catching Time of demonstration - Thursday August 24th from 2 P.M. till dark - rain date Friday August 25th. Come any time after 2 P.M. and stay as long as you like Location - Frank Reist farm between Schaefferstown and Lebanon or Route 897. Watch for signs. FREE REFRESHMENTS PARTICIPATING I.N. DEALERS SWOPE & BASHORE, INC. C. P. LONG, INC. JONESTOWN, PA. INTERNATIONAL HARVESTER SALES & SERVICE requires more development. Dr. Carlson stated, however, that “The remaining problems are technical rather than theoretical. We know what can be done using a model system. Now the crucial task is to characterize the special FRYSTOWN, PA. EPHRATA, PA. requirements of the individual species and hybrid desired from parasexual reproduction.” At Brookhaven National Laboratory, the scientists used two species of tobacco plants that had previously been hybridized |"new jXmesway | Rugged Chain Barn Cleaner j 1 Frees You Manheim Pike Lancaster, Pa. 17601 for Better Things Heavy Forged Link Chain Galvanized Steel Elevator Powerful Transmission Ph: 393-3906 Lancaster Farming Saturday, August 19,1972 — by standard genetic crosses, thus providing a basis for comparison with the hybrid produced by the parasexual method. The plants produced by both methods were identical in all respects. Using two species of tobacco Method (Nicotiana glauca and N. langsdorffii), large numbers of leaf cells were isolated and the cellulose walls which surround these cells were removed by enzymatic digestion. Many of these wall-less cells (protoplasts) were then fused following simple chemical manipulations The resulting mixed population of normal and fused protoplasts was placed in a regenerative culture medium which would only support the growth of the fused cells containing the desired hybrid genetic information. The fused cells which grew into colonies were then removed from the regenerative medium and placed in another medium completely devoid of hormones This procedure prohibits growth of tissue from the in dividual parents but allows the hybrid to form rudimentary shoots and leaves. The hybrid shoots were then grafted onto the freshly cut stems of one of the parents (N. glauca) where they grew into fertile, mature plants There plants were examined for distinguishing biochemical, cytogenetic, and morphological characteristics. The hybrid produced by the fusion of two leaf cell protoplasts resembled in every respect the hybrid between the same two species procuced by standard sexual techniques. The research on parasexual hybridization is supported principally by the Division of Biomedical and Environmental Research, U.S. Atomic Energy commission. Try A Classified Ad It Pays! ★FARM ★ ANIMAL * MWPMOIP b %V FREE! Your sale can be listed in our Sales Register without charge Just mail details to Lancaster Farming, P O. Box 266, Lititz, Pa 17543 Mow, vacuum and mulch all in one operation with the Simplicity vacuum collector that goes anywhere the tractor can Cleans full width of the mower Available in 8-10-15 HP. Models Easy Terms L. H. Brubaker, Inc. 350 Strasburg Pike Lancaster > ■ 397-5179 ** 29
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