Geneticists Perfect New Hybridization
(Continued From Page 8)
ir present concepts of world
tod production to be changed
larkedly.
The biologists emphasize that
se of parasexual hybridization
i produce new crop varieties
LANCASTER LABORATORIES, INC.
ANALYTICAL SERVICES DIVISION
Feeds, Flour, Forages, Foods
Dairy Products, Water, Waste Water
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2425 New Holland Pike
Lancaster, Pa. 17601
Telephone (717)656-9043 or
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Red Fever Demo Day:
Come and catch it!
A new Kind of fever has hit the country.
It began last summer with the introduction of IH’s new
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An interest and enthusiasm began to build that is un
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And we’d like to show you why. By letting you test drive
some of our new tractors. Under field conditions.
Discover the superior workpower our engines provide.
How the proven Hydrostatic transmission can improve
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your comfort and reduce fatigue.
Bring along-the cap you’ve been wearing in the field.
(No matter whose insignia is on it.) We’ll trade you our
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Even if you’re not ready to pick up a new tractor. Pick
up a new cap.
■ m
rorS fouEr
red fever
reu it;vci
It’s catching
Time of demonstration - Thursday August 24th from
2 P.M. till dark - rain date Friday August 25th. Come
any time after 2 P.M. and stay as long as you like
Location - Frank Reist farm between Schaefferstown
and Lebanon or Route 897. Watch for signs.
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PARTICIPATING I.N. DEALERS
SWOPE & BASHORE, INC.
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JONESTOWN, PA.
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requires more development. Dr.
Carlson stated, however, that
“The remaining problems are
technical rather than theoretical.
We know what can be done using
a model system. Now the crucial
task is to characterize the special
FRYSTOWN, PA.
EPHRATA, PA.
requirements of the individual
species and hybrid desired from
parasexual reproduction.”
At Brookhaven National
Laboratory, the scientists used
two species of tobacco plants that
had previously been hybridized
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Lancaster Farming Saturday, August 19,1972 —
by standard genetic crosses, thus
providing a basis for comparison
with the hybrid produced by the
parasexual method. The plants
produced by both methods were
identical in all respects.
Using two species of tobacco
Method
(Nicotiana glauca and N.
langsdorffii), large numbers of
leaf cells were isolated and the
cellulose walls which surround
these cells were removed by
enzymatic digestion.
Many of these wall-less cells
(protoplasts) were then fused
following simple chemical
manipulations
The resulting mixed population
of normal and fused protoplasts
was placed in a regenerative
culture medium which would
only support the growth of the
fused cells containing the desired
hybrid genetic information. The
fused cells which grew into
colonies were then removed from
the regenerative medium and
placed in another medium
completely devoid of hormones
This procedure prohibits
growth of tissue from the in
dividual parents but allows the
hybrid to form rudimentary
shoots and leaves. The hybrid
shoots were then grafted onto the
freshly cut stems of one of the
parents (N. glauca) where they
grew into fertile, mature plants
There plants were examined
for distinguishing biochemical,
cytogenetic, and morphological
characteristics. The hybrid
produced by the fusion of two leaf
cell protoplasts resembled in
every respect the hybrid between
the same two species procuced by
standard sexual techniques.
The research on parasexual
hybridization is supported
principally by the Division of
Biomedical and Environmental
Research, U.S. Atomic Energy
commission.
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Lancaster >
■ 397-5179 **
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