How can we detect and study life in soils from other planets and still remain safe from the danger posed by alien life forms? Pennsylvania State University microbiologists are at work on this problem as the U. S. space program expands the search for extraterrestrial life. At the request of the National Aeronautics and Space Ad ministration (NASA), Dr. Lester E. Casida, Jr., professor of microbiology, has begun an effort to determine if soil which has been sterilized to kill all life forms, to reduce the risk of contamination, can still yield information about the life it contained. Can we kill alien life forms, in other words, without destroying the evidence of their existence? So far, using Earth soil, Drr Casida and his assistants David, L. Balkwill and David P. Labeda, Ph.D. candidates in microb iology, College of Science, have found that one form of heat sterilization cannot be used. Dr. Casida says, “the cells are not only killed, but are totally Soil Study Of Planets Being Conducted destroyed by the process.” However, the Penn State team has found that another heat method leaves many of the dead cells at least partially intact and, Dr. Casida adds, ‘‘very preliminary work on chemical sterilization looks like it might work as well.” Dr. Casida has been studying terrestrial soil bacteria for over 15 years and has discovered Support for AEB “California Egg Producer, Jack Hayre, recently presented American Egg Board with a check for $5,000 as a token of his support for the national ad vertising and educational effort currently being undertaken by AEB. This gift was gratefully acknowledged with a Resolution of Appreciation passed by the AEB Executive Committee at its recent meeting,” so stated Ed ward D. Murphy, Chairman of AEB’s Board of Directors. “This unsolicited and volun tary investment in our national several new types of bacteria which are extremely difficult to isolate for study in the laboratory. He and his students have also developed two techniques (thin-sectioned and frozen fractured preparations) which make it possible to use the electron microscope to study bacteria as they occur naturally in the soil. Using these non destructive techniques, they have Program Grows promotional program is most welcome, and I hope indicative of the growing support on the part of the egg industry for a strong national program,” continued Chairman Murphy. While additional funds have been provided to AEB via carton manufacturers and poultry breeders, the support of the producers, packers, and distributors of eggs is also vital to the growth of these new programs being proposed by the American Egg Board. Lancaster Farming, Saturday, October 6,1973 — discovered that bacterial cysts and dwarf bacteria, too small to be seen with conventional light microscopes, frequently inhabit soil. Dr. Casida explains that the other usual techniques for studying soil bacteria require trying to make them grow in the laboratory on special media or food. He says, “these growth methods are frequently un successful since 99 jier cent of the bacteria in soil have extreme difficulty in adapting to growth under laboratory conditions.” With this fact in mind, Dr. Casida warns that the first efforts to detect life on Mars during the unmanned Viking mission in 1976 may prove disappointing. He explains that the life-detecting device that will be soft-landed on Mars will not bring any samples back to earth for study. The automated device will scoop-up and test soil for signs of life, relying completely on growth techniques or' metabolic responses, and then relay the information back to earth. Dr. Casida notes: “Since we can’t grow most of the organisms in Earth soil it should be doubly difficult to grow Mars bacteria by remote control.” He emphasizes that negative results from Viking will not in dicate a lifeless Mars but, rather, increase importance of the development of non-growth detection techniques such as the use of electron microscopy. If Dr. Casida’s current NASA funded study is successful, a soil specimen gathered from another planet may one day be sterilized in transit to kill living material before it gets to Earth. Then, with the threat of damage to life on Earth diminished, the dead bacteria can be detected and studied using Dr. Casida’s techniques. I Clay tablets dating from about 2000 B.C. show that in Babylonia valuables were deposited for a service charge of 1/16th of what they were worth. Interests on loans ran as high as 33-1/3 percent! 43